Oxidative DNA damage and disturbance of antioxidant capacity by alternariol in Caco-2 cells.
作者： Celia Fernández-Blanco , Guillermina Font , Maria-Jose Ruiz
DOI: 10.1016/J.TOXLET.2015.03.013
关键词:
摘要: Oxidative stress occurs as a consequence of an imbalance between the prooxidant/antioxidant systems, causing increase intracellular generation reactive oxygen species. Alternariol (AOH), mycotoxin produced by Alternaria sp. can alter action glutathione (GSH) and enzymes involved in redox system, damage to cellular macromolecules such DNA. The aims this work were determine induction oxidative antioxidant defenses relation (GSH), reductase (GR), transferase (GST), peroxidase (GPx) levels DNA Caco-2 cells derived from adenocarcinoma human colon. AOH was confirmed alteration GSH defense system after 15, 30 60 μM exposure during 24h. significantly decreased 43% treatment with compared control. activity GPx GR reduced 30% 23%, respectively AOH. GST increased (approximately 22%) AOH, while it comparison Analysis performed using Comet assay 24h, where % tail 70% 85%
sci-hub.se 参考文章(30)
Shao-jun He, Jia-fa Hou, Yu-yi Dai, Zhen-lei Zhou, Yi-feng Deng, N-acetyl-cysteine protects chicken growth plate chondrocytes from T-2 toxin-induced oxidative stress. Journal of Applied Toxicology. ,vol. 32, pp. 980- 985 ,(2012) , 10.1002/JAT.1697
Celia Fernández-Blanco, Guillermina Font, Maria-Jose Ruiz, Oxidative stress of alternariol in Caco-2 cells Toxicology Letters. ,vol. 229, pp. 458- 464 ,(2014) , 10.1016/J.TOXLET.2014.07.024
Pratheeba Yogendrarajah, Liesbeth Jacxsens, Sarah De Saeger, Bruno De Meulenaer, Co-occurrence of multiple mycotoxins in dry chilli (Capsicum annum L.) samples from the markets of Sri Lanka and Belgium Food Control. ,vol. 46, pp. 26- 34 ,(2014) , 10.1016/J.FOODCONT.2014.04.043
Markus Fehr, Gudrun Pahlke, Jessica Fritz, Morten O. Christensen, Fritz Boege, Martina Altemöller, Joachim Podlech, Doris Marko, Alternariol acts as a topoisomerase poison, preferentially affecting the IIα isoform Molecular Nutrition & Food Research. ,vol. 53, pp. 441- 451 ,(2009) , 10.1002/MNFR.200700379
B. Mallebrera, G. Font, M.J. Ruiz, Disturbance of antioxidant capacity produced by beauvericin in CHO-K1 cells. Toxicology Letters. ,vol. 226, pp. 337- 342 ,(2014) , 10.1016/J.TOXLET.2014.02.023
A. Prosperini, A. Juan-García, G. Font, M.J. Ruiz, Beauvericin-induced cytotoxicity via ROS production and mitochondrial damage in Caco-2 cells. Toxicology Letters. ,vol. 222, pp. 204- 211 ,(2013) , 10.1016/J.TOXLET.2013.07.005
Wafa Hassen, Imen Ayed-Boussema, Amaia Azqueta Oscoz, Adela De Cerain Lopez, Hassen Bacha, None, The role of oxidative stress in zearalenone-mediated toxicity in Hep G2 cells: Oxidative DNA damage, gluthatione depletion and stress proteins induction Toxicology. ,vol. 232, pp. 294- 302 ,(2007) , 10.1016/J.TOX.2007.01.015
U. Tiemann, W. Tomek, F. Schneider, M. Müller, R. Pöhland, J. Vanselow, The mycotoxins alternariol and alternariol methyl ether negatively affect progesterone synthesis in porcine granulosa cells in vitro Toxicology Letters. ,vol. 186, pp. 139- 145 ,(2009) , 10.1016/J.TOXLET.2009.01.014
Caroline Frizzell, Doreen Ndossi, Shewit Kalayou, Gunnar S. Eriksen, Steven Verhaegen, Morten Sørlie, Christopher T. Elliott, Erik Ropstad, Lisa Connolly, An in vitro investigation of endocrine disrupting effects of the mycotoxin alternariol Toxicology and Applied Pharmacology. ,vol. 271, pp. 64- 71 ,(2013) , 10.1016/J.TAAP.2013.05.002
Diana Dinu, Gabriela Oana Bodea, Corina Diana Ceapa, Maria Cristina Munteanu, Florentina Israel Roming, Andreea Iren Serban, Anca Hermenean, Marieta Costache, Otilia Zarnescu, Anca Dinischiotu, Adapted response of the antioxidant defense system to oxidative stress induced by deoxynivalenol in Hek-293 cells. Toxicon. ,vol. 57, pp. 1023- 1032 ,(2011) , 10.1016/J.TOXICON.2011.04.006