High-fidelity estimates of spikes and subthreshold waveforms from 1-photon voltage imaging in vivo.
作者： Karel Svoboda , Yoav Adam , Adam E. Cohen , Liam Paninski , Urs L. Böhm
DOI: 10.1016/J.CELREP.2021.108954
关键词:
摘要: Summary The ability to probe the membrane potential of multiple genetically defined neurons simultaneously would have a profound impact on neuroscience research. Genetically encoded voltage indicators are promising tool for this purpose, and recent developments achieved high signal-to-noise ratio in vivo with 1-photon fluorescence imaging. However, these recordings exhibit several sources noise signal extraction remains challenge. We present an improved pipeline, spike-guided penalized matrix decomposition-nonnegative factorization (SGPMD-NMF), which resolves supra- subthreshold voltages in vivo. method incorporates biophysical optical constraints. validate pipeline simultaneous patch-clamp from mouse layer 1 simulated composite datasets realistic noise. demonstrate applications hippocampus expressing paQuasAr3-s or SomArchon1, cortex SomArchon1 Voltron, zebrafish spines zArchon1.
sci-hub.st 参考文章(30)
Luke Campagnola, Megan B. Kratz, Paul B. Manis, ACQ4: an open-source software platform for data acquisition and analysis in neurophysiology research Frontiers in Neuroinformatics. ,vol. 8, pp. 3- 3 ,(2014) , 10.3389/FNINF.2014.00003
Eran A. Mukamel, Axel Nimmerjahn, Mark J. Schnitzer, Automated analysis of cellular signals from large-scale calcium imaging data Neuron. ,vol. 63, pp. 747- 760 ,(2009) , 10.1016/J.NEURON.2009.08.009
Ilan Lampl, Iva Reichova, David Ferster, Synchronous membrane potential fluctuations in neurons of the cat visual cortex. Neuron. ,vol. 22, pp. 361- 374 ,(1999) , 10.1016/S0896-6273(00)81096-X
Peter Szűcs, Vitor Pinto, Boris V. Safronov, Advanced technique of infrared LED imaging of unstained cells and intracellular structures in isolated spinal cord, brainstem, ganglia and cerebellum Journal of Neuroscience Methods. ,vol. 177, pp. 369- 380 ,(2009) , 10.1016/J.JNEUMETH.2008.10.024
Glenn J Goldey, Demetris K Roumis, Lindsey L Glickfeld, Aaron M Kerlin, R Clay Reid, Vincent Bonin, Dorothy P Schafer, Mark L Andermann, Removable cranial windows for long-term imaging in awake mice Nature Protocols. ,vol. 9, pp. 2515- 2538 ,(2014) , 10.1038/NPROT.2014.165
Misha B Ahrens, Michael B Orger, Drew N Robson, Jennifer M Li, Philipp J Keller, Whole-brain functional imaging at cellular resolution using light-sheet microscopy Nature Methods. ,vol. 10, pp. 413- 420 ,(2013) , 10.1038/NMETH.2434
Lin Tian, S Andrew Hires, Tianyi Mao, Daniel Huber, M Eugenia Chiappe, Sreekanth H Chalasani, Leopoldo Petreanu, Jasper Akerboom, Sean A McKinney, Eric R Schreiter, Cornelia I Bargmann, Vivek Jayaraman, Karel Svoboda, Loren L Looger, Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators Nature Methods. ,vol. 6, pp. 875- 881 ,(2009) , 10.1038/NMETH.1398
P. Jesper Sjöström, Ede A. Rancz, Arnd Roth, Michael Häusser, Dendritic Excitability and Synaptic Plasticity Physiological Reviews. ,vol. 88, pp. 769- 840 ,(2008) , 10.1152/PHYSREV.00016.2007
C. Satou, Y. Kimura, H. Hirata, M. L. Suster, K. Kawakami, S.-i. Higashijima, Transgenic tools to characterize neuronal properties of discrete populations of zebrafish neurons Development. ,vol. 140, pp. 3927- 3931 ,(2013) , 10.1242/DEV.099531
Tsai-Wen Chen, Trevor J Wardill, Yi Sun, Stefan R Pulver, Sabine L Renninger, Amy Baohan, Eric R Schreiter, Rex A Kerr, Michael B Orger, Vivek Jayaraman, Loren L Looger, Karel Svoboda, Douglas S Kim, None, Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. ,vol. 499, pp. 295- 300 ,(2013) , 10.1038/NATURE12354