Distinct reorganization of collagen architecture in lipopolysaccharide-mediated premature cervical remodeling
作者： Shanmugasundaram Nallasamy , Meredith Akins , Breanna Tetreault , Kate Luby-Phelps , Mala Mahendroo
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摘要: Previous work has identified divergent mechanisms by which cervical remodeling is achieved in preterm birth (PTB) induced hormone withdrawal (mifepristone) or lipopolysaccharide (LPS). Our current study aims to document how collagen architecture modified achieve premature mice treated with LPS as a model of infection-induced inflammation. Cervices were collected on gestation day (d) 15 from ripening and compared d15 d18 controls well PTB model. Second harmonic generation (SHG) electron microscopy utilized for visualization morphology ultrastructure. LPS-mediated characterized unique structural changes fiber morphology. treatment increased the interfibrillar spacing fibrils. A preferential disruption subepithelial region midstroma was evidenced pores lacking signal SHG images LPS-treated mice. Coinciding this alteration, infiltration neutrophils concentrated stromal midstromal implicating potential role immune cells extracellular matrix reorganization inflammation-induced ripening. The demonstrates disorganization structure
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