Regulation of ubiquitin-dependent cargo sorting by multiple endocytic adaptors at the plasma membrane

摘要: Endocytic protein trafficking is directed by sorting signals on cargo molecules that are recognized cytosolic adaptor proteins. However, the steps necessary to segregate variety of cargoes during endocytosis remain poorly defined. Using Caenorhabditis elegans, we demonstrate multiple plasma membrane endocytic adaptors function redundantly regulate clathrin-mediated and recruit components endosomal complex required for transport (ESCRT) machinery cell surface direct ubiquitin-modified substrates. Moreover, our data suggest preassembly with ESCRT-0 at enhances efficiency downstream events in endolysosomal system. In absence a heterooligomeric composed FCHO, Eps15, intersectin, accumulation diminished, degradation slows significantly without affecting rate its internalization. Consistent role ESCRT endocytosis, further show accumulates subset clathrin-coated pits human cells. Our findings unique mechanism which sequestered into pathway.