Effect of heparin on whole blood activated partial thromboplastin time using a portable, whole blood coagulation monitor.

摘要: Objectives : To evaluate the responsiveness of whole blood activated partial thromboplastin time (aPTT) to varying heparin doses in vitro and examine ex vivo relationship aPTT plasma concentration. Design Prospective, controlled laboratory study. Setting Surgical suites at a tertiary center. Patients patients volunteers center were eligible for inclusion this In evaluation was performed using specimens obtained from each five, healthy volunteers. Ex 30 cardiac surgical before after systemic administration extracorporeal circulation. Interventions Blood added syringes containing amounts unfractionated porcine evaluation. For evaluation, 20 U/kg heparin. Measurements Main Results divided into two aliquots mixing with One aliquot used measure coagulation monitor immediately collection 3 mins later, second determine conventional, laboratory-based assay. Linear regression analysis demonstrated high correlation (r = .94 ; r2 =.88) between assay systems bias mean measurement difference 1.6 secs ± 2 sD limits -15 + 18.2 secs. As indicated by comparable slopes, increasing concentration similar among individual subjects. Whole measurements specimen storage immediate measurements. aliquots. duplicate an anti-factor X active chromogenic A =.89 r =.79) observed. Conclusions Heparin aPTT, measured portable monitor, is that conventional over clinically relevant range concentrations vivo. On-site should be useful clinical situations requiring rapid results.