Dissolved carbohydrates in seawater. II, A spectrophotometric procedure for total carbohydrate analysis and polysaccharide estimation

摘要: A procedure is described which adds a hydrolysis step to the Johnson and Sieburth 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) assay for total dissolved monosaccharides. The advantages of monosaccharide test are retained in carbohydrate test, giving nearly equivalent responses variety combined carbohydrates. sample 50 ml adequate both assays permit estimation polysaccharide by difference. Values Narragansett Bay adjacent waters ranged from 452 2023 μg l−1 carbohydrate, 272 1353 polysaccharide, 153 814 monosaccharide, accounted 6–18%, 4–13%, 2–5% carbon, respectively. We suggest that this sensitive precise will be useful investigating distribution carbohydrates seawater factors affect its production, utilization.