Cloning of a new testis-enriched gene C4orf22 and its role in cell cycle and apoptosis in mouse spermatogenic cells

摘要: Spermatogenesis is a complicated and dynamic cellular differentiation process mainly regulated by genes, steroid hormones environmental factors. Although number of genes involved in spermatogenesis have been identified, there are still lot underlying remained unexplained. Here, novel gene C4orf22, also known as 1700007G11Rik or Cfap299 was identified from mouse testis. C4orf22 protein contains 233 amino acid residues highly conserved metazoan species. mRNA predominantly expressed testis increased 2-week-old testes to 8-week-old during the developing RT-PCR qRT-PCR. Immunohistochemical analysis indicated that distributed cytoplasm spermatogonia primary spermatocytes, which further confirmed C4orf22-tagged with GFP GC-1 GC-2 cells. Over-expression pEGFP-C3-C4orf22 significantly inhibited cells apoptosis promoted cell cycle progression an increase S G2 phase. Conversely, small interfering RNA (siRNA) silencing could cause arrested at G2/M Western blot qRT-PCR results showed over-expression expressions anti-apoptotic bcl-2 decreased expression caspase-3, caspase-8 Bax. Our suggest may be spermatogenesis, for first time, unravels its potential role regulating through regulatory pathway