Development of specific collagen scaffolds to support the osteogenic and chondrogenic differentiation of human bone marrow stromal cells.
作者： Jonathan I. Dawson , Denys A. Wahl , Stuart A. Lanham , Janos M. Kanczler , Jan T. Czernuszka
DOI: 10.1016/J.BIOMATERIALS.2008.03.040
关键词:
摘要: Type I Collagen matrices of defined porosity, incorporating carbonate substituted hydroxyapatite (HA) crystals, were assessed for their ability to support osteo- and chondrogenic differentiation human bone marrow stromal cells (HBMSCs). Collagen-HA composite scaffolds supported the osteogenic HBMSCs both in vitro vivo as demonstrated by histological micro-CT analyses indicating extensive penetration alkaline phosphatase expressing new matrix synthesis with localised areas immunologically positive osteocalcin. In vivo, osteoid formation implant origin was observed vasculature. Chondrogenic evidenced peripheral regions pure collagen systems an abundance Sox9 chondrocytes embedded within a proteoglycan II rich ECM. The introduction microchannels scaffold architecture seen enhance chondrogenesis. Tissue specific gene expression corresponding indicate that growth suggest potential this platform understanding ECM cues necessary osteogenesis
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