The effect of phenethyl alcohol on Bacillus subtilis transformation

摘要: Abstract Phenethyl alcohol at a concentration of 0.05 % reduces neither the growth rate Bacillus subtilis , as determined by A 630 m μ measurements, nor viable titer during 60-min exposure. When phenethyl is added concomitantly with DNA to competent B. there 50–70 inhibition transformation without any effect on cells. Treatment isolated transforming 0.5 specific biological activity, and no evidence for binding or interaction [ 14 C]phenethyl was obtained Sephadex chromatography, methylated albumin kieselguhr CsCl density gradient centrifugation. Addition after uptake completed did not inhibit transformation, potential transformants became resistant deoxyribonuclease same time. Incubation cells prior addition resulted in greater than when simultaneously DNA, if this incubation over an hour long, removal centrifugation washing restore competence. more transformants, but excess failed change number from inhibited mixture. inhibits transport radioactive initial attachment