Possible chondroprotective effect of canakinumab: An in vitro study on human osteoarthritic chondrocytes

摘要: Canakinumab is a human IgGκ monoclonal antibody that neutralizes the activity of interleukin (IL)-1β blocking interaction with IL-1β receptors. Our study aimed to evaluate in vitro effect canakinumab on osteoarthritic (OA) chondrocytes cultivated presence or absence tumor necrosis factor (TNF)-α. Articular cartilage was obtained from femoral heads patients osteoarthritis (OA). Chondrocytes were incubated two concentrations (1μg/ml and 10μg/ml) alone TNF-α (10ng/ml) for 48h. We evaluated cell viability, release proteoglycans (PG) nitric oxide (NO) culture medium, inducible synthase (iNOS) metalloproteinanes (MMP)-1,3,13 gene expression, apoptosis, morphological feature by transmission electron microscopy (TEM). did not have cytotoxic effect. Cell viability reduced significantly (p<0.001) restored at both used. determined significant decrease PG an increase NO MMP-1,3,13 expression. increased levels decreased (1μg/ml, p<0.01; 10μg/ml, p<0.01) cells cultured TNF-α. The data confirmed immunocytochemistry assay iNOS. A reduction expression induced canakinumab. experiments pro-apoptotic demonstrated protective role results concerning biochemical further findings TEM. showed counteracts negative effects OA chondrocyte cultures may potential chondroprotective OA.