Development of an Optimal Diaphragmatic Hernia Rabbit Model for Pediatric Thoracoscopic Training

摘要: Our objectives were to standarize the procedure needed reproduce a similar surgical scene which pediatric surgeon would face on repairing Bochdalek hernia in newborns and define optimal time period for development that achieve realistic scenario with minimimal animal suffering. Twenty New Zealand white rabbits weighing 3-3.5 kg divided into four groups depending frame since creation thoracoscopic repair: 48 h, 72 96 h 30 days. trigono was identified procedures repair standarized. Blood collected hematology (red blood cells, platelets, hemoglobin hematocrit), biochemistry (blood urea nitrogen, creatinine, alanine aminotransferase, aspartate lactate dehydrogenase creatine kinase) gas analysis (arterial pH, partial pressure of oxygen, carbon dioxide, oxygen saturation bicarbonate) at baseline before surgial repairment. Glucocorticoid metabolites concentration faeces measured. Thoracoscopy video recordings evaluated by six surgeons rated from 0 10 according similarities congenital diaphragmatic newborn its approach. Statistical methods included variance, comparisons between followed post-hoc Tukey's test. Fourty -eight showed be obtain point view minimal stress animals.