T- and B-Cell Epitopes in the Secreted Mycobacterium bovis Antigen MPB70 in Mice
作者： S. Tollefsen , J. M. Pollock , T. Lea , M. Harboe , H. G. Wiker
DOI: 10.1046/J.1365-3083.2003.01211.X
关键词:
摘要: MPB70 is a soluble secreted protein highly expressed in Mycobacterium bovis and strains of bacille Calmette-Guerin (BCG); as such, it candidate for subunit DNA vaccines against tuberculosis. was screened T-cell epitopes four different inbred mouse strains. Major histocompatibility complex (MHC) H-2b-expressing mice (C57BL/6) interferon-gamma (IFN-gamma) after stimulation with peptides from the regions 1-20, 41-50, 81-110, 121-150 161-193 sequence. H-2db (B6D2) splenocytes IFN-gamma some same peptides, whereas H-2d (BALB/c DBA/2) did not secrete upon peptides. Sera immunized native incomplete Freund's adjuvant (IFA), mpb70 or live BCG Moreau were found to contain antibodies antigen. IFA exhibited high titres peptide-reactive immunoglobulin G1 (IgG1) antibodies, DNA-immunized reacted IgG2a As recognized by T B cells have previously been stimulate immune responses humans, cattle rabbits, we conclude that these may be good general T- B-cell candidates vaccine broad applicability.
blackwell-synergy.com参考文章(46)
L L An, J L Whitton, A multivalent minigene vaccine, containing B-cell, cytotoxic T-lymphocyte, and Th epitopes from several microbes, induces appropriate responses in vivo and confers protection against more than one pathogen. Journal of Virology. ,vol. 71, pp. 2292- 2302 ,(1997) , 10.1128/JVI.71.3.2292-2302.1997
Jhagvaral Hasbold, Marilyn R. Kehry, Philip D. Hodgkin, Jonathan Sui-Yin Hong, Integrating signals from IFN-gamma and IL-4 by B cells: positive and negative effects on CD40 ligand-induced proliferation, survival, and division-linked isotype switching to IgG1, IgE, and IgG2a. Journal of Immunology. ,vol. 163, pp. 4175- 4181 ,(1999)
Douglas B Lowrie, Ricardo E Tascon, Vania LD Bonato, Valeria MF Lima, Lucia H Faccioli, Evangelos Stavropoulos, M Joseph Colston, Robert G Hewinson, Karin Moelling, Celio L Silva, None, Therapy of tuberculosis in mice by DNA vaccination. Nature. ,vol. 400, pp. 269- 271 ,(1999) , 10.1038/22326
Georg Widera, Melissa Austin, Dietmar Rabussay, Cheryl Goldbeck, Susan W. Barnett, Minchao Chen, Louisa Leung, Gillis R. Otten, Kent Thudium, Mark J. Selby, Jeffrey B. Ulmer, Increased DNA vaccine delivery and immunogenicity by electroporation in vivo. Journal of Immunology. ,vol. 164, pp. 4635- 4640 ,(2000) , 10.4049/JIMMUNOL.164.9.4635
Specific skin-reactive protein from culture filtrate of Mycobacterium bovis BCG. Infection and Immunity. ,vol. 31, pp. 1152- 1160 ,(1981) , 10.1128/IAI.31.3.1152-1160.1981
G A Cole, T Tao, T L Hogg, K W Ryan, D L Woodland, Binding motifs predict major histocompatibility complex class II-restricted epitopes in the Sendai virus M protein. Journal of Virology. ,vol. 69, pp. 8057- 8060 ,(1995) , 10.1128/JVI.69.12.8057-8060.1995
Monique Bachy, Florence Boudet, Michel Bureau, Yves Girerd-Chambaz, Pierre Wils, Daniel Scherman, Claude Meric, Electric pulses increase the immunogenicity of an influenza DNA vaccine injected intramuscularly in the mouse. Vaccine. ,vol. 19, pp. 1688- 1693 ,(2001) , 10.1016/S0264-410X(00)00406-0
Hirohito Naruse, Robert A. Good, Kazunori Onoé, Yasushi Itoh, Kazumasa Ogasawara, Kimitaka Takami, Toshihiko Gotohda, Determination of amino acids on agretopes of pigeon cytochrome c-related peptides specifically bound to I-A allelic products European Journal of Immunology. ,vol. 24, pp. 76- 83 ,(1994) , 10.1002/EJI.1830240113
Yael Altuvia, Ora Schueler, Hanah Margalit, Ranking potential binding peptides to MHC molecules by a computational threading approach. Journal of Molecular Biology. ,vol. 249, pp. 244- 250 ,(1995) , 10.1006/JMBI.1995.0293
Samuel M. Behar, Chris C. Dascher, Michael J. Grusby, Chyung-Ru Wang, Michael B. Brenner, Susceptibility of mice deficient in CD1D or TAP1 to infection with Mycobacterium tuberculosis. Journal of Experimental Medicine. ,vol. 189, pp. 1973- 1980 ,(1999) , 10.1084/JEM.189.12.1973