The inner centromere protein (INCENP) antigens: movement from inner centromere to midbody during mitosis.

摘要: We describe a novel set of polypeptide antigens that shows dramatic change in structural localization during mitosis. Through metaphase these define new chromosomal substructure is located between the sister chromatids. Because are concentrated pericentromeric region, we have provisionally termed them INCENPs (inner centromere proteins). The (two polypeptides 155 and 135 kD) were identified with monoclonal antibody was raised against bulk proteins mitotic chromosome scaffold fraction. These two most tightly bound known. When scaffolds prepared, 100% detectable remain associated. therefore unprepared for fate at anaphase. As chromatids separate, dissociate fully from them, remaining behind plate as migrate to spindle poles. During anaphase found on coarse fibers central spindle, also close apposition cell membrane region forming contractile ring. telophase, gradually become focused onto midbody, together which they ultimately discarded. Several possible vivo roles suggested by data: regulation chromatid pairing, stabilization plane cleavage, separation poles