Epidemiology and Molecular Characterization of Extended-Spectrum Beta-Lactamase-Producing Enterobacter spp., Pantoea agglomerans, and Serratia marcescens Isolates from a Bulgarian Hospital

摘要: Forty-two extended-spectrum beta-lactamase (ESBL)-producing isolates of Enterobacter aerogenes, cloacae, Pantoea agglomerans, and Serratia marcescens, collected consecutively during the period January-November 2011 from University Hospital in Varna, Bulgaria, were studied to characterize their ESBLs by isoelectric focusing, group-specific PCR, sequencing. The epidemiological relationship was evaluated random amplified polymorphic DNA analysis (RAPD). Transferability ESBL genes determined conjugation experiments. Plasmid done replicon typing PstI fingerprinting. overall rate production 20%. most widespread enzyme CTX-M-3, found 64%. It dominant E. aerogenes (100%) S. marcescens (83%). SHV-12, CTX-M-15 among cloacae 50%, 35%, 45%, respectively. Three main CTX-M-3-producing epidemic clones have been detected. Among isolates, six different RAPD profiles discerned. plasmids harboring blaCTX-M-3 belonged IncL/M type demonstrated similar fingerprinting profiles. IncFII detected two CTX-M-15-producing isolates. Our results demonstrate wide intrahospital dissemination clonal carrying conjugative plasmids.