Molecular Survey of β-Lactamases Conferring Resistance to Newer β-Lactams in Enterobacteriaceae Isolates from Polish Hospitals

摘要: The first national survey of resistance to newer β-lactams in nosocomial populations Enterobacteriaceae Poland was performed. study covered all nonrepetitive enterobacterial isolates cultured from specimens inpatients 13 regional secondary-care hospitals November 2003 January 2004. Among 2,388 isolates, the predominant species Escherichia coli (59.6%), followed by Proteus mirabilis (14.5%) and Klebsiella spp. (8.5%). frequency extended-spectrum β-lactamases (ESBLs) very high, with ESBLs present 11.1% 40.4% pneumoniae latter value greatly exceeding that for E. (2.5%). contribution outbreak significant, resulting, example, a particularly high rate ESBL producers among Serratia marcescens (70.8%). pool types overwhelmingly dominated (81.7%) CTX-M-like CTX-M-3 (80.6%) CTX-M-15, SHV (17.5%; SHV-2, SHV-5, SHV-12) sporadic TEM-like enzymes (0.7%; TEM-19 TEM-48) being next most frequent. Acquired AmpC-type cephalosporinases were observed exclusively P. mirabilis, 20.5% this (compared 11.5% isolates). All these (CMY-12, CMY-15, novel variant, CMY-38) originated Citrobacter freundii. Four (two isolates), K. (one isolate), isolate) produced class A inhibitor-resistant (TEM-30, TEM-32, TEM-37, SHV-49), such identified Poland. documented both specific more global characteristics epidemiology β-lactamase-mediated enterobacteria Polish demonstrated has reached an alarming level.