MLST reveals potentially high-risk international clones of Enterobacter cloacae

摘要: Objectives: To perform the first multinational Enterobacter cloacae clonality study, using MLST scheme newly developed in Japan. Methods: The analysis included 195 rectal carriage E. isolates resistant to expanded-spectrum cephalosporins (ESCs), collected from patients 12 hospital units across Europe and Israel. All of were typed by PFGE 173 subjected MLST. ESC resistance was analysed phenotypically; genes encoding ESBLs carbapenemases identified PCR sequencing. Results: distinguished 88 STs, which correlated with data. more discriminatory, producing 129 pulsotypes (169 patterns). Numerous STs observed several countries each. most widespread ST66, ST78, ST108 ST114, each having at least 10 three five countries, diversified into multiple pulsotypes, clusters related one or centres. Analysis against database revealed epidemic clonal complexes, such as those central genotypes ST74 (including ST78) ST114 ST66). equally overexpression AmpC cephalosporinase ESBL production. Among producers some spreading subclones identified, including specific ST78 associated CTX-M-15 Several produced carbapenemase VIM-1 KPC-2. Conclusions: Together information available database, our results suggest that, like Escherichia coli Klebsiella pneumoniae, harbours lineages increased potential that may be spread.