Epidemiologic and Genotypic Review of Carbapenemase-Producing Organisms in British Columbia, Canada, between 2008 and 2014

摘要: Carbapenemase-producing organisms (CPOs) are a serious emerging problem for health care facilities worldwide. Owing to their resistance most antimicrobial therapies, CPOs difficult treat and pose challenge infection prevention control. Since 2010, lab-based surveillance PCR-based testing were implemented in British Columbia (BC), Canada. A review of BC from 2008 March 2014 was done characterize the mechanisms possible clonal strain transmission compare pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), plasmid restriction fragment length polymorphism (RFLP) as molecular tools. During this study period, total 177 CPO cases identified. Patient demographics travel history reviewed, descriptive analysis carried out. PFGE profiles, MLST, RFLP subset Escherichia coli, Klebsiella pneumoniae, Enterobacter species isolates obtained analyzed. Our findings demonstrate that have been increasing number over time, 1 isolate/year retrospectively identified 2009 82 2013 30 first quarter 2014. Overall, K. pneumoniae lack clonality, although some seemingly related clusters found. Plasmid showed evidence spread plasmids carrying carbapenemase-encoding genes between examined isolates. Analysis cloacae revealed more nature these BC. The presence provides interpatient organism both within institutions. Although our study, NDM-harboring E. appeared clonally, carbapenem seems be mediated.