Propagation of an infertile hermaphrodite mouse lacking germ cells by using nuclear transfer and embryonic stem cell technology

摘要: Animals generated by systematic mutagenesis and routine breeding are often infertile because they lack germ cells, maintenance of such lines animals has been impossible. We found a hermaphrodite mouse in our colony, genetic male with an abnormal Y chromosome lacking developing cells. tried to clone this conventional nuclear transfer but without success. ES cells produced from blastocysts, which had cloned using somatic cell (ntES cells) mouse, were also unable produce offspring when injected into enucleated oocytes. Although we able two chimeric these ntES tetraploid complementation, infertile, lacked However, normal diploid many produced. One transmitted genes fertile daughters via X chromosome-bearing sperm. Thus, used propagate mice