Establishment of Embryonic Stem Cell Lines from Adult Somatic Cells by Nuclear Transfer
作者： T WAKAYAMA
DOI: 10.1016/B978-012164730-8/50011-3
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摘要: Publisher Summary This chapter discusses how to establish embryonic stem cell lines from adult somatic cells by nuclear transfer. The ntES differentiate not only all three germ layers in vitro, but also vivo. Some nuclei can develop full term after a second techniques could be useful for research tools used reprogramming, imprinting, and gene modification. Thaw the freezing long-term culture medium (KSOM) inside refrigerator overnight. When preparing micromanipulation pipette manipulation chamber, make sure that diameter of outer is smaller than oocyte. Break tip at flat, vertical, blunt ends using microforge. A notched often kills oocyte during enucleation injection. Collect oocyte/cumulus complex ampullae oviduct 14–15 h hCG injection move into hyaluronitase (0.1%) containing CZB–HEPES medium.
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