Efficient Establishment of Mouse Embryonic Stem Cell Lines from Single Blastomeres and Polar Bodies

摘要: Recently, ES cell lines were established from single blastomeres taken eight-cell embryos in mice and humans with success rates of 4% 2%, respectively, which suggests that the method could be used regenerative medicine to reduce ethical concerns over harm embryos. However, those studies other cells as supporting cells. Here, we report a simple highly efficient establishing mouse blastomeres, are simply plated onto feeder layer embryonic fibroblasts modified medium. A total 112 two-cell (establishment rate, 50%–69%), early four-cell (28%–40%), late (22%), (14%–16%) stage We also successfully 18 parthenogenetic first (36%–40%) second polar bodies (33%), nuclei reconstructed by nuclear transfer. Most examined maintained normal karyotypes expressed markers pluripotency, including germline transmission chimeric mice. Our results suggest all early-stage or have potential converted into without any special treatment. Disclosure conflicts interest is found at end this article.