Establishment of Male and Female Nuclear Transfer Embryonic Stem Cell Lines from Different Mouse Strains and Tissues

摘要: Nuclear transfer can be used to generate embryonic stem cell lines from somatic cells, and these have great potential in regenerative medicine. However, it is still unclear whether any individual or type such lines. Here, we tested seven different male female mouse genotypes three types as sources of nuclei determine the efficiency establishing nuclear Lines were successfully established all sources. Cumulus F(1) showed a significantly higher cumulative establishment rate reconstructed oocytes than other cells; however, there no genotype differences success rates cloned blastocysts. Thus, overall depends on preimplantation development, and, once embryos reached blastocyst stage, disappear. All that demonstrated at least one line subsequently contributed germline transmission chimeric mice, so clearly possess same cells derived fertilized embryos. generated relatively easily variety inbred both sexes, even though may more difficult clones directly.