Ammonia concentration in bovine follicular fluid and its effect during in vitro maturation on subsequent embryo development.
作者： D.S Hammon , S Wang , G.R Holyoak
DOI: 10.1016/S0378-4320(99)00069-X
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摘要: Abstract Ammonia concentration in bovine follicular fluid (bFF) at different developmental stages and the effects on oocyte maturation vitro were investigated this study. In Experiment 1, 80 bFF samples collected from follicles four size categories ( 10 mm) to determine ammonia concentration. was greater P mm follicles, respectively. 2, abattoir oocytes n =2885) matured media containing concentrations of 0 μM (Control), 29 (A1), 88 (A2), 132 (A3), 176 (A4) 356 (A5), followed by fertilization culture. There no difference >0.05) cleavage rates morula or blastocyst development between treatments, indicating that exposure does not adversely influence subsequent embryonic development.
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