Role of cytokines in cancer cachexia in a murine model of intracerebral injection of human tumours.

摘要: Abstract To study the role of cytokines that are relevant in cancer cachexia syndrome due to intracerebral tumours, mice were injected with human A431 epidermoid carcinoma, OVCAR3 ovarian carcinoma and GBLF glioma cells comparing (i.c.) systemic (i.p. or s.c.) routes implantation. Anorexia weight loss developed within 7–10 days i.c. well before a large tumour developed, while i.c.-injected did not induce until day 20, when was large. By contrast, i.p. s.c. tumours without evidence anorexia. Thus, intracerebrally-growing resulted independent mass, we investigated their cytokine pattern. Serum levels murine predictive development. Reverse-transcriptase polymerase chain reaction (RT-PCR) analysis revealed brain tumour-bearing expression interleukin-(IL-)1α, IL-1β LIF all samples IL-6 two four animals IL-6, detected necrosis factor-α (TNFα) samples. Only expressed brains cells. Murine increased only A431-bearing mice. simultaneously monoclonal antibody (mAb) directed against receptor cells, but those mAb showed significant increase survival time partial temporary attenuation symptoms. These results suggest model may be important cachectogenic factor centrally released by even limited number