Activation of JNK/SAPK in primary glial cultures: II. Differential activation of kinase isoforms corresponds to their differential expression.

摘要: Recently, we reported on the activation of c-Jun N-terminal kinase (JNK) in primary glial cells noting certain differences patterns astrocytes and oligodendrocytes (Zhang et al., J Neurosci Res 46:114–121;1996). In this extended study, have examined expression levels JNK1 JNK2 isoforms different cell types including two vitro-defined astroglial subtypes (type-1 type-2), microglia. An in-gel assay extracts JNK-immunoprecipitates revealed both type-1 response to TNFα, microglia, TNFα bacterial lipopolysaccharide. The strong JNK microglia contrasted with a predominant over type-2 oligodendrocytes, sharing common lineage. Immunoblot immunocytochemical analyses using isoform-speciflc antibodies showed differential thereby accounting for their observed activation.