DNA polymerase III from Saccharomyces cerevisiae. II. Inhibitor studies and comparison with DNA polymerases I and II.

摘要: The newly identified yeast DNA polymerase III was compared to polymerases I and II the mitochondrial polymerase. Inhibition by aphidicolin (I50) of I, II, 4, 6, 0.6 micrograms/ml, respectively. enzyme insensitive drug. N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate strongly inhibited (I50 = 0.3 microM), whereas less sensitive 80 microM). Conditions that allowed proteolysis proceed during preparation extracts converted from a form 2.4 microM) resistant 2 mM). is greater than 5 With most other inhibitors tested (N-ethylmaleimide, heparin, salt) only small differences were observed between three nuclear polymerases. Polyclonal antibodies did not inhibit nor those recognized Western blotting. Monoclonal crossreact with III. results show distinct II.