Characterization and genetic distance analysis of cassava ( Manihot esculenta Crantz) germplasm from Mozambique using RAPD fingerprinting

作者: A.M. Zacarias , A.-M. Botha , M.T. Labuschagne , I.R.M. Benesi

DOI: 10.1023/B:EUPH.0000047057.05642.74

关键词:

摘要: Twenty-eight cassava genotypes from Mozambique, along with seven Angola, Madagascar, Nigeria, Togo, Columbia, and Thailand for comparison, were fingerprinted using random amplified polymorphic DNA (RAPD) analysis. The Mozambican material represented a wide range of landraces. A total 311 scored RAPD loci used to calculate genetic distances between the genotypes. This revealed an average distance 3.1% all germplasm. Mozambiquen was 2.7%, whilst accessions other countries showed 3.4%. Neighbor-joining (NJ) method cluster analysis yielded tree that did not indicate relationship geographic distribution diversity.

参考文章(7)
D.L. Jennings, C.H. Hershey, Cassava breeding: a decade of progress from international programmes Progress in Plant Breeding–1. pp. 89- 116 ,(1985) , 10.1016/B978-0-407-00780-2.50008-4
Stephen L. Dellaporta, Jonathan Wood, James B. Hicks, A plant DNA minipreparation: Version II Plant Molecular Biology Reporter. ,vol. 1, pp. 19- 21 ,(1983) , 10.1007/BF02712670
John R Beeching, Philippe Marmey, Marie-Christine Gavalda, Michel Noirot, Howard R Haysom, Monica A Hughes, Andre Charrier, An Assessment of Genetic Diversity Within a Collection of Cassava (Manihot esculenta Crantz) Germplasm Using Molecular Markers Annals of Botany. ,vol. 72, pp. 515- 520 ,(1993) , 10.1006/ANBO.1993.1139
David J. Rogers, Henry S. Fleming, A monograph ofManihot Esculenta with an explanation of the taximetrics methods used Economic Botany. ,vol. 27, pp. 1- 113 ,(1973) , 10.1007/BF02862222
A. C. Roa, M. M. Maya, M. C. Duque, J. Tohme, A. C. Allem, M. W. Bonierbale, AFLP analysis of relationships among cassava and other Manihot species Theoretical and Applied Genetics. ,vol. 95, pp. 741- 750 ,(1997) , 10.1007/S001220050620
Philippe Marmey, John R. Beeching, Serge Hamon, Andr� Charrier, Evaluation of cassava ( Manihot esculenta Crantz) germplasm collections using RAPD markers Euphytica. ,vol. 74, pp. 203- 209 ,(1994) , 10.1007/BF00040402
A. A. Myburg, A-M. Botha, B. D. Wingfield, W. J. M. Wilding, Identification and Genetic Distance Analysis of Wheat Cultivars using RAPD Fingerprinting Cereal Research Communications. ,vol. 25, pp. 875- 882 ,(1997) , 10.1007/BF03543891