Simultaneous determination of nucleobases, nucleosides and saponins in Panax notoginseng using multiple columns high performance liquid chromatography

摘要: Abstract A new multiple columns HPLC method for simultaneous determination of 16 characteristic components, 5 nucleobases and nucleosides (uracil, cytidine, uridine, guanosine adenosine), 11 saponins (notoginsenoside R1, ginsenoside Rg1, Re, notoginsenoside R4, Fa, Rb1, R2, Rg2, Rh1, Rd K), in the root Panax notoginseng, a valued traditional Chinese medicinal herb, were developed. Notoginsenoside Fa K first quantitatively determined P. notoginseng. The compounds separated on Zorbax SB-Aq column (150 × 4.6 mm, 5.0 μm) analyzed using Bonus-RP with switching. temperature was set at 30 °C. Mobile phase composed 5 mM ammonium acetate aqueous (A), water (B) acetonitrile (C) gradient elution. flow rate 1.5 mL/min detection wavelengths 260 nm nucleosides, 203 nm saponins. developed had good repeatability sensitivity quantification analytes overall precision (including intra- inter-day) less than 3% (RSD), LOD LOQ 1.33 μg/mL 5.12 μg/mL, respectively. successfully applied to 15 samples notoginseng collected from different places China, which indicated that can be used comprehensive quality control