Reversible Inhibition of Poliovirus RNA Synthesis In Vivo and In Vitro by Viral Products
作者： Anton Röder , Klaus Koschel
DOI: 10.1128/JVI.14.4.846-852.1974
关键词:
摘要: In poliovirus-infected HeLa-S3 cells, the protease inhibitors tolylsulfonyl-phenylalanyl chloromethyl ketone and iodoacetamide cause an accumulation of large precursor proteins, they block viral RNA synthesis most probably via these products. Viral polymerase activity can, however, be extracted by detergent containing buffer (Tris/Nonidet P-40, deoxycholate) from inhibited cells. Only cytoplasmic extracts infected cells treated with or contain a protein which inhibits in vitro reaction.
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