Salt-Dependent Conditional Protein Splicing of an Intein from Halobacterium salinarum.
作者： Julie N. Reitter , Christopher E. Cousin , Michael C. Nicastri , Mario V. Jaramillo , Kenneth V. Mills
DOI: 10.1021/ACS.BIOCHEM.6B00128
关键词:
摘要: An intein from Halobacterium salinarum can be isolated as an unspliced precursor protein with exogenous exteins after Escherichia coli overexpression. The promotes splicing and uncoupled N-terminal cleavage in vitro, conditional on incubation NaCl or KCl at concentrations of >1.5 M. reaction also is reduction a disulfide bond between two active site cysteines. Conditional under these relatively mild conditions may lead to advances intein-based biotechnology applications hints the possibility that this H. could serve switch control extein activity physiologically relevant conditions.
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