Orthodontic tooth movement in alveolar cleft repaired with a tissue engineering bone: an experimental study in dogs.

摘要: Tissue engineering approaches have been successfully used in repairing bone defects and become a viable alternative to autologous bone. The aim of the present study was investigate if construct porous beta-tricalcium phosphate (β-TCP) combined with osteogenically induced marrow stromal cells (bMSCs) could repair alveolar cleft, allow for subsequent orthodontic tooth movement canine model. Twelve osteotomy surgeries six animals were made bilaterally randomly implanted by (1) tissue-engineered complex bMSCs/β-TCP (group A, n=4), (2) β-TCP alone B, (3) obtained from iliac C, n=4). Contralateral created one animal left untreated serve as blank control observe spontaneous healing defects. Sequential fluorescent labeling radiographic observation evaluate new formation mineralization each defect. Orthodontic initiated 8 weeks after surgical operation 12 weeks, then dogs sacrificed histological histomorphometric analysis. Results indicated that dramatically promoted achieved favorable height repaired when compared alone, which absorbed severely. overall effect equivalent bone; physiological function restored allowing adjacent teeth move into newly formed grafted region. This demonstrated tissue combination bMSCs is feasible clinical approach patients cleft movement.