Confirmation of a Link between Fungal Pigmentation, Turgor Pressure, and Pathogenicity Using a New Method of Turgor Measurement

摘要: Money, N. P., and Howard, R. J. 1996. Confirmation of a link between fungal pigmentation, turgor pressure, pathogenicity using new method measurement.Fungal Genetics Biology20,217–227. A novel approach to measurement has confirmed melanin synthesis appressorial pressure in the rice blast fungusMagnaporthe grisea.The technique employs cold stage measure melting point ice within individual cells. Turgor is then calculated from difference points intracellular fluid surrounding cell. Conidia ofM. griseawere incubated on various surfaces, appressoria developed 6 h. Measurements conidia showed that wild-type melanin-deficient strains generated same pressures, which decreased before germ tube emergence. By contrast, much higher pressures than buf−mutant. These experiments strengthen hypothesis confers by facilitating development high appressoria.