Unique tissue-specific level of DNA nucleotide excision repair in primary human mammary epithelial cultures

摘要: DNA repair is essential for the maintenance of genomic integrity and stability. Nucleotide excision (NER) a major pathway responsible remediation damage caused by UV light, bulky adducts, cross-linking agents. We now show that NER capacity differentially expressed in human tissues. established primary cultures peripheral blood lymphocytes (PBLs: N = 33) foreskin fibroblasts (FF: 6), as well adult breast tissue (N 22) using unique culture system, measured their unscheduled synthesis (UDS) functional assay. Relative to FF, cells exhibited only 24.6 ± 2.1% PBLs 8.9 1.2%. Cells from therefore have distinctive capacity. The capacities all three cell types had similar coefficients variation range 10%–15%, which should be taken into account when running controls this contextual Unlike previous studies speculation field, we found was not affected morphology, donor age, or proliferation S phase index. While transformed lymphoblastoid line TK6 within our PBL samples, tumor-derived MDA MB-231 four-fold higher than normal tissue. These analysis baseline critical basis evaluation effects “mutator” genes etiological factors development cancer.