In situ localization of beta-glucans in the cell wall of Schizosaccharomyces pombe.

摘要: The chemical composition of the cell wall Sz. pombe is known as β-1,3-glucan, β-1,6-glucan, α-1,3-glucan and α-galactomannan; however, three-dimensional interactions those macromolecules have not yet been clarified. Transmission electron microscopy reveals a three-layered structure: outer layer electron-dense, adjacent less dense, third bordering membrane dense. In intact cells pombe, high-resolution scanning microscope surface completely filled with α-galactomannan particles. To better understand organization to complement our previous studies, we set out locate three different types β-glucan by immuno-electron microscopy. Our results suggest that dense contains mainly β-1,6-branched β-1,3-glucan. Occasionally line gold particles can be seen, labelling fine filaments radiating from layer, suggesting some radial contain β-1,6-glucan preferentially located underneath layer. Linear β-1,3-glucan exclusively in primary septum dividing cells. only labels secondary does co-localize linear while present both septa. early stages formation persists until formed; then just before division label disappears. From these involved perhaps separation two daughter addition, frequently found on Golgi apparatus, small vesicles membrane. These give fresh evidence for hypothesis synthesized endoplasmic reticulum–Golgi system exported Copyright © 2001 John Wiley & Sons, Ltd.