Motor Coordination via a Tug-of-War Mechanism Drives Bidirectional Vesicle Transport

摘要: The microtubule motors kinesin and dynein function collectively to drive vesicular transport. High-resolution tracking of vesicle motility in the cell indicates that transport is often bidirectional, characterized by frequent directional changes. However, mechanisms coordinating collective activities oppositely oriented bound same cargo are not well understood. To examine motor coordination, we purified neuronal vesicles analyzed their via automated particle with nanometer resolution. reconstituted vitro closely models movement LysoTracker-positive primary neurons, where processive bidirectional interrupted switches, diffusional movement, pauses. Quantitative analysis copurify a low number stably motors: one five four motors. These observations compare predictions from stochastic tug-of-war model, driven force-dependent kinetics teams opposing absence external regulation. Together, these indicate move robustly small complement tightly suggest an efficient regulatory scheme for changes engaged manifest large cargo.