Complex interaction between dengue virus replication and expression of miRNA-133a

摘要: Dengue virus (DENV) is the most common vector-borne viral infection worldwide with approximately 390 million cases and 25,000 reported deaths each year. MicroRNAs (miRNAs) are small non-coding RNA molecules responsible for regulation of gene expression by repressing mRNA translation or inducing degradation. Although miRNAs possess antiviral activity against many mammalian-infecting viruses, their involvement in DENV replication poorly understood. Here, we explored relationship between cellular microRNAs using bioinformatics tools. We overexpressed miRNA-133a Vero cells to test its role analyzed RT-qPCR. Furthermore, polypyrimidine tract binding protein (PTB), a involved replication, was western blot. In addition, profiled challenged DENV-2, Taqman miRNA. Bioinformatic analysis revealed that 3' untranslated region (3'UTR) genome all four serotypes targeted several miRNAs, including miRNA-133a. found overexpression synthetic suppressed replication. Additionally, observed PTB transcription , target, down-regulated during infection. Based our results propose 3'UTR down-regulates endogenous first hours regulates possibly through modulation host factor such as PTB. Further investigations needed verify whether has an anti-DENV effect vivo.