Functional analysis of Epinephelus coioides peroxisome proliferative-activated receptor α (PPARα): Involvement in response to viral infection.

摘要: Abstract Peroxisome proliferative–activated receptor α (PPARα) belongs to the superfamily of nuclear receptors (NR). Studies have demonstrated that PPARα functions in energy metabolism, hepatic function, immune response, cell cycle, and apoptosis. In teleost fish, few studies investigated role response. this study, grouper gene (EcPPARα) was for its viral infection. The open reading frame EcPPARα encoded a protein 469 amino acids contained an N-terminal domain (NTD), DNA-binding (DBD), hinge region, C-terminal ligand-binding (LBD). Phylogenetic analysis revealed most closely related homologous genes Sander lucioperca Perca flavescens. Upon challenge with SGIV (Singapore iridovirus) RGNNV (Red-spotted nervous necrosis virus), expression levels were significantly upregulated different tissues. Subcellular localization showed localized throughout cytoplasm nucleus diffuse intracellular patterns, which is consistent pattern mammalian PPARs. Based on morphological observation cytopathic effect (CPEs), mRNAs, virus titer assays, results presented here overexpression promoted production spleen cells. Overexpression inhibited several cytokines, including interferon-related (IFN-γ, ISG15, MXI, MXII, MAVS MDA5), inflammatory cytokines (IL-1β, IL-6, IL-8, TNF-α) Toll like adaptors (TRAF6 MyD88). Luciferase activity IFN-α, IFN-γ, ISRE NF-κB promoters also decreased Due these detected play important antiviral against grouper, we speculated promotion replication may be caused by down-regulation interferon addition, through apoptotic body observation, capspase-3 detection, flow cytometry analysis, it found SGIV-induced apoptosis fathead minnow (FHM) These data increase understanding fish responses