Production of a novel bioflocculant MNXY1 by Klebsiella pneumoniae strain NY1 and application in precipitation of cyanobacteria and municipal wastewater treatment

作者: M. Nie , X. Yin , J. Jia , Y. Wang , S. Liu

DOI: 10.1111/J.1365-2672.2011.05080.X

关键词:

摘要: Aims:  To isolate and characterize the novel bioflocculant-producing bacteria, to optimize bioflocculant production evaluate its potential applications. Methods Results: Klebsiella pneumoniae strain NY1, a bacterium that produces (MNXY1), was selected on chemically defined media. It classified according 16S rRNA gene sequence, morphological microscopic characteristics. MNXY1 characterized contain 26% protein 66% total sugar. The constituent sugar monomers of MNXY1, revealed by NMR analysis, are glucose, galactose quinovose. Favourable culture conditions for were determined. Strain NY1 high level (14.9 g l−1) MNXY1. is thermostable tolerant extreme pH. precipitated 54% cyanobacteria from laboratory 72% suspended solids raw wastewater. Conclusions:  identified produce outstanding performance in practical applications availability copious amounts make it attractive further investigation development industrial scale applications. Significance Impact Study:  This first report identification quinovose-containing application protein–polysaccharide complex precipitation cyanobacteria. These findings suggest holds great use management harmful algae city wastewater treatment.

参考文章(58)
H. Salehizadeh, S.A. Shojaosadati, Isolation and characterisation of a bioflocculant produced by Bacillus firmus Biotechnology Letters. ,vol. 24, pp. 35- 40 ,(2002) , 10.1023/A:1013853115624
H Salehizadeh, S.A Shojaosadati, Extracellular biopolymeric flocculants. Recent trends and biotechnological importance. Biotechnology Advances. ,vol. 19, pp. 371- 385 ,(2001) , 10.1016/S0734-9750(01)00071-4
Ryuichiro Kurane, Kazuki Toeda, Kiyoshi Takeda, Tomoo Suzuki, Culture Conditions for Production of Microbial Flocculant by Rhodococcus erythropolis Agricultural and biological chemistry. ,vol. 50, pp. 2309- 2313 ,(1986) , 10.1080/00021369.1986.10867747
L. Guo, Doing Battle With the Green Monster of Taihu Lake Science. ,vol. 317, pp. 1166- 1166 ,(2007) , 10.1126/SCIENCE.317.5842.1166
Michel. DuBois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, Fred. Smith, Colorimetric Method for Determination of Sugars and Related Substances Analytical Chemistry. ,vol. 28, pp. 350- 356 ,(1956) , 10.1021/AC60111A017
Shu-Guang Wang, Wen-Xin Gong, Xian-Wei Liu, Lin Tian, Qin-Yan Yue, Bao-Yu Gao, Production of a novel bioflocculant by culture of Klebsiella mobilis using dairy wastewater Biochemical Engineering Journal. ,vol. 36, pp. 81- 86 ,(2007) , 10.1016/J.BEJ.2007.02.003
W. Dermlim, P. Prasertsan, H. Doelle, Screening and characterization of bioflocculant produced by isolated Klebsiella sp. Applied Microbiology and Biotechnology. ,vol. 52, pp. 698- 703 ,(1999) , 10.1007/S002530051581