Regulation of the low density lipoprotein receptor and hydroxymethylglutaryl coenzyme A reductase genes by protein kinase C and a putative negative regulatory protein.
作者： J. H. Auwerx , A. Chait , S. S. Deeb
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摘要: Transcription of the low density lipoprotein receptor (LDL-R) and 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase genes was rapidly transiently induced (8.5- 2.3-fold, respectively) early during phorbol 12-myristate 13-acetate (PMA)-induced macrophage differentiation human monocytic leukemia cell line THP-1. The levels mRNA coding for LDL-R HMG-CoA increased soon after induction, reached a maximum (12- 7-fold increase, in 2-3 hr, then returned to constitutive observed before induction. stability did not change significantly differentiation, whereas that decreased by about 5-fold 6 hr addition PMA. Transcriptional induction both (5.6- 2-fold, also when undifferentiated cells were treated with cycloheximide (CHX), resulting transient increase steady-state (7- 3-fold, respectively). These results suggest expression two is maintained at uninduced THP-1 protein short half-life. Superinduction occurred PMA CHX added simultaneously. mRNAs mediated kinase C. It hypothesized C acts directly or indirectly inactivate labile negative regulatory protein. Induction hepatocarcinoma Hep G2 CHX, suggesting this mechanism regulation may exist several types.
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