BIRTH OF PIGLETS DERIVED FROM IN VITRO FERTILIZATION OF PIG OOCYTES MATURED IN VITRO
作者： M. Yoshida , Y. Mizoguchi , K. Ishigaki , T. Kojima , T. Nagai
DOI: 10.1016/0093-691X(93)90232-T
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摘要: Abstract Follicular oocytes collected from prepubertal gilts at a local slaughterhouse were matured for 36 hours in mTLP-PVA medium with pig follicular fluid (pFF) fraction and cysteine, fertilized (mTALP-PVA) vitro. After 12 of insemination, total 78% ( 14 18 ) the was vitro normal fertilization rate 43% 6 ). Of 356 embryos developed (mKRB) vitro, 84 (24%) cleaved to 2- 4-cell stage by after insemination. embryos, 75 subsequently transferred into oviducts recipient gilt. The farrowed 3 healthy piglets 117 days embryo transfer. results indicate that derived pFF cysteine can develop term.
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