Fatty acid biosynthesis during the life cycle of Debaryomyces etchellsii
作者： Fatma Arous , Tahar Mechichi , Moncef Nasri , George Aggelis
DOI: 10.1099/MIC.0.000298
关键词:
摘要: Fatty acid biosynthesis during the life cycle of ascomycetous yeast Debaryomyces etchellsii cultivated on a non-fermentable substrate, i.e. glycerol, in nitrogen rich media (NRM) and limited (NLM) has been studied. Although considerable activities key lipogenic enzymes, such as ATP citrate lyase (ACL) malic enzyme (ME), were detected vegetative cells asexual proliferation (which occurred first growth stages both NRM NLM), lipid accumulation was restricted due to high NAD+-isocitrate dehydrogenase (NAD+-ICDH). A similar enzymatic profile found ascii free ascospores produced NRM; thus low. On contrary, very ACL ME low NAD+-ICDH NLM resulting accumulation. Neutral lipids (NL) predominant fraction cellular NLM. other hand, phospholipids (P) major polar while glycolipids (G) synthesized proportions. During transition from sexual phase, percentage NL increased with significant decrease P and, lesser extent, G. High quantities linoleic esterified lipids, especially P, stage growth, while, few exceptions, stage, concentration decreased markedly, mainly oleic increased.
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