Polyclonal nature of the intestinal mucosal lymphocyte populations in inflammatory bowel disease

摘要: Abstract To define the clonality of intestinal lymphocytes involved in immune response inflammatory bowel disease, we performed a molecular genetic analysis arrangement immunoglobulin and antigen-specific T-cell receptor genes isolated lamina propria derived from resected specimens 12 patients with Crohn's 5 chronic ulcerative colitis, 7 other gastrointestinal diseases. The sensitivity this technique is sufficient to detect monoclonal population when there as little 1% clonal expansion mixed cell population. In all these groups patients, deoxyribonucleic acid non-T cells demonstrated only germ-line gene pattern, no non-germ-line rearrangements assessed by an immunoglobulin-joining heavy-chain probe. Deoxyribonucleic T showed rearrangement pattern β- γ-chain that characteristic polyclonal T-constant T-joining γ-gene probes. These results indicate non-T- T-lymphocyte populations disease controls are polyclonal.