Severe impact of hemolysis on stability of phenolic compounds

摘要: Eugenie-Raphaelle Berube has obtained a Bachelor of Science in Biochemistry from Universite du Quebec Montreal. She previously worked at the St-Lawrence Center Environment, Canada, conducting biomarker analysis to measure impact contaminants on aquatic species. been working bioanalysis industry for past 8 years Algorithme Pharma, CRO located Laval, becoming scientist bioanalytical method development quantitation pharmaceuticals biological fluids. The presence hemolyzed plasma samples can negatively preclinical and clinical sample analysis. During morphine, post-extracted instability issues were encountered human when compared with nonhemolyzed (called normal simplicity). Investigation revealed that methemoglobin using high pH reconstitution solution led degradation morphine over time. probably results radical oxidation ionized phenolic group promoted by methemoglobin. Pseudomorphine, product oxidative dimerization was observed as one products plasma. This hypothesis extended raloxifene, another phenol-containing compound. On other hand, no detected drug bearing masked phenol or carboxylic acid functionality. issue resolved below pKa moiety.