Characterization of fractionated human diploid fibroblast cell populations.
作者： Y Mitsui , EL Schneider , None
DOI: 10.1016/0014-4827(76)90236-6
关键词:
摘要: Abstract Human diploid cell populations were fractionated on the basis of size by gravity sedimentation. This separation procedure yielded that enriched for both large volumes and slow and/or non-replicating cells (cells which did not have labeled nuclei after a 48 h incubation with [ 3 H]TdR). It also small rapidly replicating nuclei). However, upon reintroduction to tissue culture conditions, lost their properties soon resembled unfractionated cultures at similar levels in vitro passage.
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