Detection of the VP6 gene of group F and G rotaviruses in broiler chicken fecal samples from the Amazon region of Brazil
作者： Joana D. P. Mascarenhas , Delana A. M. Bezerra , René R. Silva , Mayara J. M. Silva , Edivaldo C. Sousa Júnior
DOI: 10.1007/S00705-016-2882-Z
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摘要: The aim of this study was to detect rotavirus F (RVF) and G (RVG) in fecal specimens broiler chickens Brazil. During 2008 2011, a total 85 samples were collected. viral genome extracted, followed by polyacrylamide gel electrophoresis (PAGE), reverse transcription polymerase chain reaction (RT-PCR), nucleotide sequencing. Samples screened for rotaviruses PAGE, RVF RVG banding patterns not seen. Using RT-PCR, it found that 9.4 % (8/85) the pools contained RVF, whereas 10.6 % (9/85) RVG. predicted amino acid sequences from Brazilian 94.4-95.7 % 96.8-96.9 % identical, respectively, those prototypes Germany. detection provides important epidemiological data about simultaneous circulation affecting flocks Amazon region
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