SSU1-R, a sulfite resistance gene of wine yeast, is an allele of SSU1 with a different upstream sequence

摘要: Abstract A sulfite resistance gene, SSU1-R, was cloned from a highly sulfite-resistant strain of Saccharomyces cerevisiae, Y-9, which isolated as contaminant wine must. The coding region sequence SSU1-R almost identical to that SSU1, the gene has been shown be responsible for resistance. Although SSU1 is located on chromosome XVI S. upstream homologous with VIII. In our Northern analysis, an intense band detected in Y-9 and K1-V1116 strains, both exhibit strong resistance, without medium. On other hand, under same experimental conditions, no OC-2, exhibits weak Physical mapping SSU1/SSU1-R showed this VIII two WE14. OC-2 twenty-three wine, sake, beer, shochu (Japanese distilled liquor), alcohol, bakery, laboratory had XVI, four strains it chromosomes. Thus, difference seems cause differences transcription rates degree