Polyhydroxylalkyleneamines: A class of hydrophilic cationic polymer-based gene transfer agents

摘要: Abstract A new series of cationic polymers, poly[ N , -bis-(2-hydroxylpropyl) alkylalcoholamine- co -ethylenediamine] were synthesized by directly cross-linking several dichloro alkylating agents with ethylenediamine and its derivatives. Co-polymerization cystamine introduces biodegradable disulfide bonds to the polymer backbone. When tested on COS-1 cells, PHAs showed reduced cytotoxicity, broad DNA ratios, enhanced transfection activity that was 2–9-fold better than polyethylenimine. Comparison studies also revealed chemical physical parameters related biological activities these polymers. The length side chain groups affects both toxicity polymers; a group moderate size appeared be optimal for high low toxicity. Introduction backbone further polymer. Fractionated molecular weight ≥ 5000 Da equally effective but smaller polymers ineffective in transfection. This flexible synthesis route enables determination key structural could aid improvement polymer-based agents.