Three new piscidins from orange-spotted grouper (Epinephelus coioides): Phylogeny, expression and functional characterization
作者： Ze-rui Zhuang , Xiao-dong Yang , Xia-zi Huang , Hui-xian Gu , Han-yin Wei
DOI: 10.1016/J.FSI.2017.04.011
关键词:
摘要: The present study reports the identification, and characterization of three new putative piscidin paralogues, ecPis-2, ecPis-3 ecPis-4, from orange-spotted grouper (Epinephelus coioides). cDNA piscidins with 207, 216, 231 nt open reading frame encoded respectively a 68-, 71-, 76-amino acid preprotein consisting predicted signal peptide, mature peptide prodomain. phylogenetic analysis indicated that multiple paralogues in one fish species are highly diversified, suggested should be family belonging to superfamily ancient cationic, linear, amphipathic host defence peptides widespread across invertebrate vertebrate taxa comprising insect cecropins ceratotoxins, amphibian dermaseptins. synthetic peptides, ecPis-2S, ecPis-3S ecPis-4S, had strong activities against bacterial fungal species. EcPis-3S exhibited powerful activity infective stage Cryptocaryon irritans, theronts. full length ecPis-2 ecPis-4 by removal ecPis-2L ecPis-4L respectively, potency bacterial, parasitic ecPis-2S was proved exist spleen HPLC followed ESI-LCMS analysis. Basal transcriptions were detected not only potential sites pathogen entry such as gills, skin intestine, but also tissues head kidney, trunk blood cells, abundant immune however different expressed constitutively levels tissues. In addition, expression upregulated challenged Vibrio Parahaemolyticus, at time point after bacteria injection. These results support being important immune-related genes innate system playing multifunctional complementary roles following their structural functional diversification, pattern difference. Finally, this facilitates evaluation 2L, ecPis-3S, -4L templates therapeutic agents pathogens.
sci-hub.se 参考文章(34)
B. G. Kapoor, V. J. Smith, J. M. O. Fernandes, G. Zaccone, A. García-Ayala, J. Meseguer, Antimicrobial peptides of the innate immune system. Science Publishers, Inc.. pp. 241- 275 ,(2008)
Umaporn Silphaduang, Edward J. Noga, Peptide antibiotics in mast cells of fish Nature. ,vol. 414, pp. 268- 269 ,(2001) , 10.1038/35104690
Noriaki Iijima, Norio Tanimoto, Yohko Emoto, Yohko Morita, Kazumasa Uematsu, Tomoya Murakami, Toshihiro Nakai, Purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major FEBS Journal. ,vol. 270, pp. 675- 686 ,(2003) , 10.1046/J.1432-1033.2003.03419.X
Dorjee G. Tamang, Milton H. Saier, Jr., The Cecropin Superfamily of Toxic Peptides Journal of Molecular Microbiology and Biotechnology. ,vol. 11, pp. 94- 103 ,(2006) , 10.1159/000092821
Yu-Hsuan Jheng, Lin-Han Lee, Chen-Hung Ting, Chieh-Yu Pan, Cho-Fat Hui, Jyh-Yih Chen, Zebrafish fed on recombinant Artemia expressing epinecidin-1 exhibit increased survival and altered expression of immunomodulatory genes upon Vibrio vulnificus infection. Fish & Shellfish Immunology. ,vol. 42, pp. 1- 15 ,(2015) , 10.1016/J.FSI.2014.10.019
Zhi-Xin Yin, Wei He, Wei-Jian Chen, Jiang-Hui Yan, Jun-Ning Yang, Siu-Ming Chan, Jian-Guo He, Cloning, expression and antimicrobial activity of an antimicrobial peptide, epinecidin-1, from the orange-spotted grouper, Epinephelus coioides Aquaculture. ,vol. 253, pp. 204- 211 ,(2006) , 10.1016/J.AQUACULTURE.2005.10.002
X.M. Dan, A.X. Li, X.T. Lin, N. Teng, X.Q. Zhu, A standardized method to propagate Cryptocaryon irritans on a susceptible host pompano Trachinotus ovatus Aquaculture. ,vol. 258, pp. 127- 133 ,(2006) , 10.1016/J.AQUACULTURE.2006.04.026
Jianyi Yang, Renxiang Yan, Ambrish Roy, Dong Xu, Jonathan Poisson, Yang Zhang, The I-TASSER Suite: protein structure and function prediction Nature Methods. ,vol. 12, pp. 7- 8 ,(2015) , 10.1038/NMETH.3213
Jian-Shan Bai, Ming-Quan Xie, Xing-Quan Zhu, Xue-Ming Dan, An-Xing Li, Comparative studies on the immunogenicity of theronts, tomonts and trophonts of Cryptocaryon irritans in grouper Parasitology Research. ,vol. 102, pp. 307- 313 ,(2007) , 10.1007/S00436-007-0766-6
Jorge Masso-Silva, Gill Diamond, Antimicrobial Peptides from Fish Pharmaceuticals. ,vol. 7, pp. 265- 310 ,(2014) , 10.3390/PH7030265