Method of Cleaning and/or Sanitizing a Separation Matrix

摘要: The present invention concerns a method of cleaning and/or sanitizing separation matrix comprising multimers immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to porous support. comprises the steps of: a) optionally purifying mixture first immunoglobulin using matrix; b) providing liquid at least 50% by volume an aqueous alkali metal hydroxide solution; and c) contacting with for predetermined contact time. comprise mutants parental Fc-binding domain Staphylococcus (SpA), as defined SEQ ID NO 51 or 52, wherein amino acid residues positions 13 44 52 are asparagines asparagine residue position 3 has been mutated selected from group consisting glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine arginine.