Primary human muscle precursor cells obtained from young and old donors produce similar proliferative, differentiation and senescent profiles in culture

摘要: The myogenic behaviour of primary human muscle precursor cells (MPCs) obtained from young (aged 20-25 years) and elderly people 67-82 was studied in culture. Cells were compared terms proliferation, DNA damage, time course extent marker expression during differentiation, fusion, size the formed myotubes, secretion regulatory cytokine TGF-β1 sensitivity to treatment. No differences observed between people. cell populations expanded culture until replicative senescence. Cultures that maintained their initial proportion (desmin positive) with passaging (n = 5) same individuals non-senescent state. senescent exhibited a greater number damage (γ-H2AX positive), showed impaired markers fused less well, smaller myotubes secreted more TGF-β. data strongly suggest MPCs have similar behaviour.