A systems-based investigation into vitamin D and skeletal muscle repair, regeneration, and hypertrophy

摘要: Skeletal muscle is a direct target for vitamin D. Observational studies suggest that low 25[OH]D correlates with functional recovery of skeletal following eccentric contractions in humans and crush injury rats. However, definitive association yet to be established. To address this gap knowledge relation damage repair, randomised, placebo-controlled trial was performed 20 males insufficient concentrations serum 25(OH)D (45 ± 25 nmol/l). Prior 6 wk supplemental D3 (4,000 IU/day) or placebo (50 mg cellulose), participants × 10 damaging the knee extensors, peak torque measured over 7 days recovery. Parallel experimentation using isolated human muscle-derived myoblast cells from biopsies 14 (37 11 nmol/l) were subjected mechanical wound injury, which enabled corresponding vitro regeneration, hypertrophy presence absence 100 nmol 1α,25(OH)2D3. Supplemental increased improved at 48 h postexercise. In vitro, 1α,25(OH)2D3 cell migration dynamics resulted myotube fusion/differentiation biochemical, morphological, molecular level together postdamage. Together, these preliminary data are first characterize role D regeneration maintaining may beneficial enhancing reparative processes potentially facilitating subsequent hypertrophy.